General Cell Lines and Cell Cultures
ATCC pRS424 phagemid; in E. coli
This is a YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. It contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. It also contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the number is about 20 per haploid cell. In the range of 1.5 +/? 1.7% of progeny per doubling. The order of the major features in this plasmid is: ori(fi) ? lacZ ? T7 promoter ? MCS (KpnI-SacI) ? T3 promoter ? lacI - ori (pMB1) ? ampR ? ori(2 micron) ? TRP1.
ATCC Paenibacillus validus
Paenibacillus validus strain Q1 is a whole-genome sequenced bacterium that was isolated from soil in Spain. This product can be used as a quality control strain.
ATCC Rothia amarae
This strain of Rothia amarae was isolated from an air sample from environmental monitoring study. This whole-genome sequenced bacterium is propagated aerobically in trypticase soy medium.
ATCC Neisseria gonorrhoeae
Resistant to spectinomycin [actinospectacin]. Quality control organism for Difco spectinomycin differentiation disks.
ATCC Lactobacillus amylovorus
Lactobacillus amylovorus strain BT1507 is a bacterium that was isolated from swine feces. This strain is cited to have applications in producing feed supplements for animals.
ATCC Klebsiella pneumoniae
Klebsiella pneumoniae strain UMJMH14 is a whole-genome sequenced bacterium that was isolated from a clinical specimen. This strain produces a low level of AmpC ?-lactamase and it can be used as a control isolate for the AmpC disk test.
ATCC Salmonella enterica; subsp. enterica; serovar Saintpaul
Salmonella enterica subsp. enterica serovar Saintpaul strain 127 was isolated from a case of cystitis in Panama. This strain is serotype 1,4,5,12:e,h:1,2 and has applications in enteric disease research.
ATCC Human papilloma virus; type 16 genome; purified phagemid DNA
pHPV-16 purified plasmid DNA is a clone of HPV16 that has been linearized at the unique BamHI site and cloned into the XhoI site of the pBluescript SK- vector. This product has applications for molecular biology.
ATCC Escherichia coli; Quantitative DNA
Quantitative Genomic DNA from Escherichia coli strain 2002-3211 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Eurofins Discoverx Ready-to-Assay EP2 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS185LRTA)
The Ready-to-Assay EP2 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER2 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER2, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
